Title:	Andiroba Oil (Carapa guianensis Aublet) Gel-Based Nanoemulsion : Development, Characterization, and In Vitro Evaluation in Wound Healing-Related Cells
Authors:	Silva, Vitória Regina Pereira da Silva, Atailson Oliveira da Rodrigues , Mosar Corrêa Py-Daniel, Karen Rapp Gomes, Bruna Rafaela Bezerra Damas, Elysa Beatriz de Oliveira Nogueira, Thalya Soares Ribeiro Vieira, Ivo José Curcino Castro, Mariana de Souza Sousa, Marcelo Henrique Carneiro, Marcella Lemos Brettas Joanitti, Graziella Anselmo
metadata.dc.identifier.orcid:	https://orcid.org/0000-0001-7434-6491 https://orcid.org/0000-0003-1210-8329 https://orcid.org/0000-0002-6888-1284
metadata.dc.contributor.affiliation:	University of Brasilia, Laboratory of Bioactive Compounds and Nanobiotechnology (LBCNano) University of Brasilia, Green Nanotechnology Group University of Brasilia, Laboratory of Bioactive Compounds and Nanobiotechnology (LBCNano) University of Brasilia, Laboratory of Bioactive Compounds and Nanobiotechnology (LBCNano) University of Brasilia (UnB), Institute of Biological Sciences, Department of Cell Biology, Laboratory of Protein Chemistry and Biochemistry University of Brasilia, Laboratory of Bioactive Compounds and Nanobiotechnology (LBCNano) State University of Northern Fluminense, Laboratory of Chemical Sciences(LCQUI) State University of Northern Fluminense, Laboratory of Chemical Sciences(LCQUI) University of Brasilia, Institute of Biological Sciences, Department of Cell Biology, Laboratory of Protein Chemistry and Biochemistry, University of Brasilia, Green Nanotechnology Group University of Brasilia, Laboratory of Bioactive Compounds and Nanobiotechnology (LBCNano), Graduation Program in Nanoscience and Nanobiotechnology University of Brasilia, Laboratory of Bioactive Compounds and Nanobiotechnology (LBCNano), Program in Pharmaceutical Science and Post-Graduation, Program in Nanoscience and Nanobiotechnology
Issue Date:	2-Oct-2026
Publisher:	American Chemical Society (ACS)
Citation:	SILVA, Vitória R. P. et al. Andiroba oil (*Carapa guianensis* Aublet) gel-based nanoemulsion: development, characterization, and In Vitro evaluation in wound healing-related cells. ACS Omega, 2026, p. A–N. DOI: https://doi.org/10.1021/acsomega.6c02635. Disponível em: https://doi.org/10.1021/acsomega.6c02635. Acesso em: 9 out. 2026.
Abstract:	Andiroba oil (Carapa guianensis Aublet) is widely used in traditional medicine due to its anti-inflammatory and reported wound-healing properties. In this study, we developed and characterized andiroba oil-based nanoemulsions (NeAnd) and gel-based nanostructured formulation (NeAndG) to investigate their physicochemical stability and initial in vitro biological effects in wound healing-related cells. Chemical characterization by GC-MS confirmed the presence of limonoids (including gedunin and its derivatives), β-sitosterol, and oleic acid in andiroba oil composition, compounds associated with anti-inflammatory and regenerative properties. The nanoemulsions exhibited hydrodynamic diameters <200 nm, low polydispersity indices (<0.3), and a negative surface charge, indicating a homogeneous and stable nanostructured system. After incorporation into the Carbopol hydrogel, the nanodroplets maintained nanoscale size while exhibiting a markedly increased negative ζ potential (≈ −74 mV), suggesting enhanced colloidal stability. Long-term stability assays demonstrated that the gel matrix significantly improved thermal stability and prevented nanodroplet aggregation compared with the nanoemulsion alone during storage (4, 25, and 37 °C for 120 days). In vitro assays using human keratinocytes (HaCaT) and fibroblasts (HGF) showed a dose-dependent decrease in cell viability. Scratch assay analysis revealed no significant enhancement of cell migration compared with controls, suggesting that the biological activity of andiroba oil in this context may involve mechanisms other than cell migration. Overall, these results demonstrate that the andiroba oil nanogel represents a stable and biocompatible nanoplatform suitable for further investigation in wound healing-related studies.
metadata.dc.description.unidade:	Instituto de Ciências Biológicas (IB) Departamento de Genética e Morfologia (IB GEM) Faculdade de Ciências da Saúde (FS) Departamento de Farmácia (FS FAR)
metadata.dc.description.ppg:	Programa de Pós-Graduação em Nanociência e Nanobiotecnologia Programa de Pós-Graduação em Ciências Farmacêuticas
Licença::	This article is licensed under CC-BY 4.0
DOI:	https://doi.org/10.1021/acsomega.6c02635
Appears in Collections:	Artigos publicados em periódicos e afins

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