Title:	Ru(II)-diphosphine/N,S-mercapto complexes and their anti-melanoma properties
Authors:	Silva, Nádija N. P. da Silva Mello, Marcos Vinicius Palmeira Acésio, Nathália O. Moraes, Carlos A. F. Araújo Neto, João Honorato de Castellano, Eduardo E. Tavares, Denise C. Oliveira, Katia Mara de Batista, Alzir A.
metadata.dc.identifier.orcid:	https://orcid.org/0000-0003-2550-5315 https://orcid.org/0000-0002-1127-6083 https://orcid.org/0000-0003-4749-0281 https://orcid.org/0000-0002-4671-2754
metadata.dc.contributor.affiliation:	Federal University of São Carlos, Departament of Chemistry Federal University of São Carlos, Departament of Chemistry University of Franca Federal University of São Carlos, Departament of Chemistry Federal University of São Carlos, Physics Institute Federal University of São Carlos, Physics Institute University of Franca Federal University of São Carlos, Departament of Chemistry
Assunto::	Complexos de rutênio(II) Atividade antitumoral Melanoma
Issue Date:	2025
Publisher:	Royal Society of Chemistry
Citation:	SILVA, Nádija N. P. da et al. Ru(ii)-diphosphine/N,S-mercapto complexes and their anti-melanoma properties. Dalton Transactions, [S. l.], v. 54, n. 2, p. 605-615, 2025. DOI: https://doi.org/10.1039/d4dt02575j.
Abstract:	We have synthesized and characterized a novel series of ruthenium complexes with formulas [RuCl(N–S)(dppm)2]PF6 (Ru1), [Ru(N–S)(dppm)2]PF6 (Ru2), [Ru(N–S)(dppe)2]PF6 (Ru3), [Ru(N–S)(dppen)2]PF6 (Ru4), [Ru(N–S)(bpy)2]PF6 (Ru5). In these formulas, N–S or S represents H2mq (2-mercapto-4(3H)-quinazoline); dppe (1,2′-bis(diphenylphosphine)ethane), dppm (1,1′-bis(diphenylphosphine)methane), or dppen (1,2′-bis(diphenylphosphine)ethene); and bpy refers to 2,2′-bipyridine. We have also compared the cytotoxicity of cisplatin with these ruthenium complexes to murine melanoma cells (B16-F10), human melanoma cells (A-375), and the non-tumoral human keratinocyte cell line (HaCat). All the ruthenium complexes inhibited melanoma cell growth in a dose-dependent manner. [Ru(2mq)(dppen)2]PF6 was four times more active toward A-375 cells than toward HaCat cells, inhibited colony formation in HaCat and A-375 cells (with a more pronounced effect on A-375 cells), altered A-375 cell morphology, and inhibited cell migration at 0.2 and 0.4 μM. In addition, we investigated how these ruthenium complexes interact with biomolecules such as DNA and Human Serum Albumin (HSA). All the ruthenium complexes interacted weakly with DNA, possibly through the grooves. Based on fluorescence assays, the ruthenium complexes interacted moderately with HSA. In light of these results, ruthenium complexes bearing phosphine and H2mq display promising cytotoxic properties against melanoma.
metadata.dc.description.unidade:	Instituto de Química (IQ)
DOI:	https://doi.org/10.1039/d4dt02575j
metadata.dc.relation.publisherversion:	https://pubs.rsc.org/dt/article/54/2/605/847525/Ru-ii-diphosphine-N-S-mercapto-complexes-and-their
Appears in Collections:	Artigos publicados em periódicos e afins

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