Título:	Análise de mirnas circulantes identificados em pacientes com carcinoma de células escamosas em cavidade oral e orofaringe
Autor(es):	Abreu, Anna Karolina De Carvalho
Orientador(es):	Ramos, Doralina do Amaral Rabello
Assunto:	Sequenciamento de nova geração (NGS) Câncer oral Orofaringe Biópsia líquida
Data de publicação:	29-Jun-2026
Data de defesa:	29-Jan-2024
Referência:	ABREU, Anna Karolina De Carvalho. Análise de mirnas circulantes identificados em pacientes com carcinoma de células escamosas em cavidade oral e orofaringe. 2024. 76 f., il Tese (Doutorado em Ciências Médicas) — Universidade de Brasília, Brasília, 2024.
Abstract:	Background Squamous Cell Carcinoma (SCC) is the most frequent cancer in the oral and/or oropharyngeal cavity and, due to its high incidence and mortality rates, is considered a significant public health issue, thus presenting distinct diagnosis and treatment challenges. In this study, circulating miRNAs associated with oral cavity and oropharyngeal SCC were identified, and further investigation was performed on their expression levels in silico, prediction of their target genes, and identification of the biological pathways they regulate.Methods and Results The RNA-seq technique for comprehensive miRNA (miRNoma) identification was applied to 16 plasma samples, including 12 from patients before initiating any treatment, 02 collected after radiotherapy in the same patient group, and 02 from the control group. After applying criteria for miRNA selection, a total of 444 miRNAs were identified from the miRNome experiment, with 339 miRNAs detected in the case group (262 in individuals before initiating any treatment and 77 in individuals after radiotherapy) and 105 in the control group. The bioinformatic analyses using independent samples from public databases enabled the identification of six prominent miRNAs significantly associated with oral cavity and oropharyngeal SCC: miR-30d-5p, miR-122-5p, miR-126-3p, miR-150-5p, miR-191-5p, and miR-223-3. A total of 194 target genes, predicted for these miRNAs, were identified using bothTargetScan and miRDB prediction platforms. A functional analysis of these genes, using the DAVID tool, highlighted pathways, and annotations with significant enrichment. Among them, the pathway microRNAs in cancer, from the Kyoto Encyclopedia of Genes and Genomes (KEGG) was enriched. In the Gene Ontology (GO) analysis, the nucleus (Cell Compartment), protein binding (Molecular Function), and negative regulation of transcription DNA-templated (Biological Process) annotations were enriched. In addition, the Protein-Protein Interaction Network analysis with STRING and Cytoscape allowed the identification of six proteinsrelevant to the studied disease: PRKCA, RRAS2, RASA1, CALML4, PAX5 and FOXO1.Conclusion Among the complex network of miRNAs associated with oral cavity and oropharyngeal SCC, our miRNome experiment, besides the in silico prediction of target genes, functional analyses, and construction of the PPI network of specific miRNAs, highlighted both the miR-150-5p and miR-223-3p as being associated with oral and oropharyngeal cancer, providing important insights into how they can modulate the expression and function of genes in this disease. These results lay the foundation for further investigation, including studies to validate the potential of these miRNAs as diagnostic or prognostic biomarkers, or as targets for the development of novel therapeutic approaches.
Unidade Acadêmica:	Faculdade de Medicina (FM)
Informações adicionais:	Tese (doutorado) — Universidade de Brasília, Faculdade de Medicina, Programa de Pós-Graduação em Ciências Médicas, 2024.
Programa de pós-graduação:	Programa de Pós-Graduação em Ciências Médicas
Aparece nas coleções:	Teses, dissertações e produtos pós-doutorado

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