Título:	Microencapsulação e nanoencapsulação como estratégias para viabilizar a utilização de bioativos extraídos da casca de Spondias mombin L.
Autor(es):	Brito, Giovanna Oliveira de
Orientador(es):	Gris, Eliana Fortes
Assunto:	Cajá Cerrados - Brasil Compostos fenólicos Câncer - tratamento Nanopartículas
Data de publicação:	25-Jun-2026
Data de defesa:	31-Jan-2023
Referência:	BRITO, Giovanna Oliveira de. 2023. Microencapsulação e nanoencapsulação como estratégias para viabilizar a utilização de bioativos extraídos da casca de Spondias mombin L.124 f., il. Dissertação (Mestrado em Ciências e Tecnologias em Saúde) — Universidade de Brasília, Ceilândia, 2023.
Abstract:	Taperebá is a cerrado fruit, rich in phenolic compounds with therapeutic potential. A challenge for using these compounds is their instability as well as their low bioavailability, and encapsulation is a tool that can minimize these issues. The present study aimed to encapsulate phenolic compounds extracted from Taperebá peel, through the development of microparticles (MPs) and nanostructured lipid carriers (PLNs), evaluate characteristics and stability of MPs and CLNs, as well as the in vitro toxicity of the CLNs in Murino melanoma cells. The quantification of total polyphenols (PT) of the extract was performed by the Folin-Ciocalteau method, and antioxidant activity, by DPPH and ABTs. The MPs were obtained by the chitosan spray drying method and characterized on encapsulation efficiency, scanning electronic microscopy (SME) morphology, and phenolic content stability stored in different conditions for 60 days. The CLNs were elaborated by the phase reversal temperature technique, with and without natural analog deep eutentic solvents (NADES). CLNs were characterized regarding encapsulation efficiency, colloidal properties, morphology by electronic transmission microscopy (MET), differential thermal analysis (DTA), thermogravimetric analysis (TGA), colloidal stability and phenolic content at different temperatures over time. The feasibility of melanoma cells with the treatment of CLNs from the MTT method was evaluated. The extract obtained the amount of PT of ~ 550 mg EAG/100 g of fresh bark and antioxidant activity of ~ 50 µmteac/g and ~ 100 µmteac/g of bark, for DPPH and ABTs. Microencapsulation obtained efficiency of 55.4%, as well as better thermal stability compared to the lyophilized extract. Nanoencapsulation obtained ~ 85% efficiency. The CLNs presented PDI values, size, and zeta potential suitable for a more stable formulation, and without NADES showed more organized morphology compared to with NADES. DTA demonstrated possible extract encapsulation, while TGA showed the largest thermal stability of the CLNs with extract compared to the lyophilized extract. The CLNs demonstrated good colloidal stability after 60 days at room and stressful temperature. The verified values of PT retention in the CLNs with NADES were considered high, with better retention in ambient temperatures and refrigerated after 60 days. The CLNs without NADES showed lower PT stability after freezing and stressful temperature. CLNS with extract and without the Nades had cytotoxic activity for B16-F10 and less toxicity in non-tumor cells
Unidade Acadêmica:	Faculdade de Ciências e Tecnologias em Saúde (FCTS) – Campus UnB Ceilândia
Informações adicionais:	Dissertação (mestrado) — Universidade de Brasília, Faculdade de Ceilândia, Programa de Pós-Graduação em Ciências e Tecnologias em Saúde, 2023.
Programa de pós-graduação:	Programa de Pós-Graduação em Ciências e Tecnologias em Saúde
Agência financiadora:	Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Aparece nas coleções:	Teses, dissertações e produtos pós-doutorado

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