Título:	Caracterização enzimática e proteômica do secretoma de cocultivo entre Trichoderma reesei rut-c30 e Pleurotus citrinopileatus em bagaço de cana-de-açúcar com avaliação do potencial lignocelulolítico
Autor(es):	Peixoto, Alencar da Silva
Orientador(es):	Ferreira Filho, Edivaldo Ximenes
Assunto:	Pectina Cana-de-açúcar - bagaço Bioconversão Fungos Biorrefinarias
Data de publicação:	19-Jun-2026
Data de defesa:	6-Mar-2026
Referência:	PEIXOTO, Alencar da Silva. Caracterização enzimática e proteômica do secretoma de cocultivo entre Trichoderma reesei rut-c30 e Pleurotus citrinopileatus em bagaço de cana-de-açúcar com avaliação do potencial lignocelulolítico. 2026. 176 f., il. Dissertação (Mestrado em Biologia Microbiana) — Universidade de Brasília, Brasília, 2026.
Abstract:	Lignocellulosic biomass from agroindustrial residues, such as sugarcane bagasse, is an abundant feedstock for second-generation biorefineries and has potential for biofuels and bioproducts. The complex matrix of cellulose, hemicellulose, lignin, and pectin in plant cell walls, however, presents physicochemical barriers to bioconversion. Notably, this study highlights the underrated role of pectin in cell wall organization and accessibility, which affects enzymatic exposure to structural polymers. In contrast to previous research efforts, this study explored whether interspecific fungal coculture could uniquely modulate the secretome and enhance hydrolytic performance on sugarcane bagasse. Based on an initial screening of Trichoderma reesei RUT-C30 with other saprotrophic fungi, with each fungal combination functionally characterized for CMCase, mannanase, pectinase, and xylanase activities, the T. reesei RUT-C30 and Pleurotus citrinopileatus coculture was selected, based on promising enzyme activities, for extended characterization. Novel parameters included assessment of pH influence, bagasse hydrolysis, and proteomic analysis by LC–MS/MS. Importantly, while coculture cellulolytic, xylanolytic, and mannanolytic activities matched those of T. reesei monoculture, pectinolytic activity was consistently elevated in coculture, as confirmed in 15-day assays, with significant differences by day 12. We observed new temporal activity patterns: early xylanolytic followed by later cellulolytic and mannanolytic increases. Coculture at pH 6 yielded peak enzyme performance, with bagasse hydrolysis maintained. Proteomics revealed 305 proteins, including diverse CAZymes, and identified a large abundance of pectinolytic enzymes and accessory proteins from P. citrinopileatus targeting pectic polysaccharides. Although quantitative comparisons of treatments are unavailable, these findings provide the first mechanistic insights into pectinolytic profiles driven by coculture. Overall, our study uniquely demonstrates selective secretome reorganization along the pectinolytic axis, suggesting that targeting pectin can optimize biomass accessibility. This framework opens up opportunities for quantitative proteomics and multi-omics, guiding the design of fungal consortia for biorefineries.
Unidade Acadêmica:	Instituto de Ciências Biológicas (IB) Departamento de Biologia Celular (IB CEL)
Informações adicionais:	Dissertação (mestrado) — Universidade de Brasília, Departamento de Biologia Celular, Programa de Pós-Graduação em Biologia Microbiana, 2026.
Programa de pós-graduação:	Programa de Pós-Graduação em Biologia Microbiana
Agência financiadora:	Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) , Fundação de Apoio à Pesquisa do Distrito Federal (FAPDF) e Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Aparece nas coleções:	Teses, dissertações e produtos pós-doutorado

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