Título:	Caracterização química e biológica de Peptídeos Antimicrobianos Curtos (SPAMs) associados a um motivo de Ligação Amino Terminal Cobre e Níquel (ATCUN)
Autor(es):	Vasconcelos, Isadora Alves de
Orientador(es):	Pires Júnior, Osmindo Rodrigues
Assunto:	Resistência bacteriana Atividade antifúngica Peptídeos antimicrobianos Citotoxicidade
Data de publicação:	18-Jun-2026
Data de defesa:	24-Fev-2025
Referência:	VASCONCELOS, Isadora Alves de.Caracterização química e biológica de peptídeos antimicrobianos curtos (spams) associados a um motivo de ligação amino terminal cobre e níquel (atcun). 2025. 63 f., il. Tese (Doutorado em Biologia Animal) — Universidade de Brasília, Brasília, 2025.
Abstract:	Antimicrobial peptides have emerged as a potential new treatment due to their ability to exploit weaknesses present in antibiotic resistance mechanisms observed in bacteria. In addition, recent studies have demonstrated that when conjugated to an Amino Terminal Copper and Nickel Binding Motif (ATCUN), antimicrobial peptides exhibit enhaced antimicrobial activity. In the present work, analogues of antimicrobial peptides with and without the addition of an ATCUN motif were chemically and biologically characterized. All peptides were able to inhibit the growth of the bacterial species evaluated with relatively low minimum inhibitory concentrations (MICs), and the four peptides conjugated to the ATCUN motif, when tested for improving their efficiency on the same bacteria, demonstrated an increase in activity antimicrobial up to eight times. The eight peptides were also able to inhibit the growth of at least one of the three strains of the genus Candida tested and, one of them, was able to increase the fungicidal activity against strains of C. albicans and C. krusei when compared to its analogue without the motif. The peptides demonstrated little cytotoxicity, with a preference for neoplastic cell lines, and did not demonstrate hemolytic activity. Regarding their secondary structures, the peptides were able to structure themselves into a α-helix when in the presence of SDS and TFE. It is the understood, from this work, that the analogues of the described antimicrobial peptides have potent bactericidal activity against microorganisms and that the association of these peptides with an ATCUN motif iscapable of increasing their bactericidal activity by two to eight times, depending on the peptide. Furthermore, it can also be observed that the peptides have antifungal and antiproliferative activity on neoplastic cells, with a possible enhacement of these activities by peptides containing the oxidizing motif.
Unidade Acadêmica:	Instituto de Ciências Biológicas (IB)
Informações adicionais:	Tese (doutorado) — Universidade de Brasília, Instituto de Ciências Biológicas, Programa de Pós-Graduação em Biologia Animal, 2025.
Programa de pós-graduação:	Programa de Pós-Graduação em Biologia Animal
Agência financiadora:	Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) , Fundação de Apoio à Pesquisa do Distrito Federal (FAPDF)
Aparece nas coleções:	Teses, dissertações e produtos pós-doutorado

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