Title:	Avaliação in vivo do potencial imunomodulador de Beta-glucana de Auricularia auricula em modelo murino de infecção tipo sepse
Authors:	Viana, Jesse Pereira Machado
Orientador(es)::	Bocca, Anamélia Lorenzetti
Coorientador(es)::	Maciel, Márcia Cristina Gonçalves
Assunto::	Sepse Beta-glucana Imunomodulação Auricularia auricula
Issue Date:	4-Mar-2026
Data de defesa::	10-Mar-2025
Citation:	VIANA, Jesse Pereira Machado. "avaliação in vivo do potencial imunomodulador de beta-glucana de auricularia auricula em modelo murino de infecção tipo sepse. 2025. 71 f., il. Dissertação (Mestrado em Biologia Molecular) — Universidade de Brasília, Brasília, 2025.
Abstract:	Sepsis is defined as life-threatening organ dysfunction caused by a deregulated host response to infection. This condition is potentially fatal and treatment challenging, requiring alternative approaches that can reduce the burden of the infectious agent and modulate the patient's immune response. In this context, β-glucans have emerged as potential candidates due to their biological activities, especially their immunomodulatory and antimicrobial activity. Objective: To investigate the effect of treatment with β-glucan from Auricularia auricula in a lethal sepsis-type systemic infection model. Methods: Chapter I - An integrative literature review based on systematic steps was carried out, with searches conducted in the PubMed, ScienceDirect, Scopus, Web of Science and Embase databases. The searches were conducted using keywords and descriptors controlled by the Medical Subject Headings (MeSH) and the uncontrolled terms “glucan,” “sepsis,” and “cecal ligation and puncture,” along with the Boolean operators “AND” and “OR,” adapting them to each specific database when necessary. The Rayyan platform was used to organize the articles obtained and to help exclude articles that did not meet the selection criteria. Chapter II (Microbial Pathogenesis will be submitted) - A preparation containing β-glucan from the basidiomycete Auricularia auricula was administered via gavage for 15 days before inducing sepsis, at a dose of 5 mg/kg as a form of prophylactic treatment. The female mice (C57BL/6) were divided into 3 groups: SHAM (“false-operated”), CLP (sepsis induced by the cecal ligation and perforation model - no treatment) and CLP GLU (sepsis induced by the CLP model, and treatment administered by gavage 15 days before the induction of sepsis). Results: Chapter I - In the majority of studies, the main type of glucan investigated was β-glucan (88.2%), a reduction in inflammatory cytokines (TNF-α and IL-6), an increase in antioxidant activity, a reduction in the occurrence of tissue damage, leading to an increase in animal survival were described. Chapter II - Treatment with β-glucan stimulates the proliferation of circulating lymphocytes and granulocytes in the blood, attenuates lung damage caused by sepsis, reduces CFU in the peritoneum and blood, prevents the occurrence of thrombocytopenia, regulates serum cytokine levels and prolongs the survival of septic mice. Conclusions: Taken together, the data presented in Chapters I and II indicate that β-glucan is a nutraceutical that represents a new therapeutic strategy with the potential to modulate the immune response, control microbial proliferation and improve survival in a state of severe sepsis induced in mice. β-glucan can be considered a therapeutic alternative to stimulate and/or modulate the immune response in cases of sepsis, and can also act as an adjuvant treatment to the use of standard drugs in the clinic, thus contributing to a better prognosis in lethal sepsis.
metadata.dc.description.unidade:	Instituto de Ciências Biológicas (IB) Departamento de Biologia Celular (IB CEL)
Description:	Dissertação (mestrado)—Universidade de Brasília, Instituto de Ciências Biológicas, Departamento de Biologia Celular, Programa de Pós-Graduação em Biologia Molecular, 2025.
metadata.dc.description.ppg:	Programa de Pós-Graduação em Biologia Molecular
Licença::	A concessão da licença deste item refere-se ao termo de autorização impresso assinado pelo autor com as seguintes condições: Na qualidade de titular dos direitos de autor da publicação, autorizo a Universidade de Brasília e o IBICT a disponibilizar por meio dos sites www.unb.br, www.ibict.br, www.ndltd.org sem ressarcimento dos direitos autorais, de acordo com a Lei nº 9610/98, o texto integral da obra supracitada, conforme permissões assinaladas, para fins de leitura, impressão e/ou download, a título de divulgação da produção científica brasileira, a partir desta data.
Agência financiadora:	Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Appears in Collections:	Teses, dissertações e produtos pós-doutorado

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