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dc.contributor.authorAraújo, Joabe Limapt_BR
dc.contributor.authorSantos, Alexandre Silvapt_BR
dc.contributor.authorSilva, Vitória Regina Miranda Carvalhopt_BR
dc.contributor.authorSantos, Lucas Carvalho dospt_BR
dc.contributor.authorMariano, André de Lima e Silvapt_BR
dc.contributor.authorFlorêncio, Isadorapt_BR
dc.contributor.authorBáo, Sônia Nairpt_BR
dc.contributor.authorSilva, Sebastião William dapt_BR
dc.contributor.authorSouza, Paulo Eduardo Narcizopt_BR
dc.contributor.authorAzevedo, Ricardo Bentespt_BR
dc.contributor.authorMuehlmann, Luís Alexandrept_BR
dc.date.accessioned2026-08-12T11:16:22Z-
dc.date.available2026-08-12T11:16:22Z-
dc.date.issued2026-6-23pt_BR
dc.identifier.citationARAÚJO, Joabe Lima; SANTOS, Alexandre Silva; SILVA, Vitória Regina Miranda Carvalho; SANTOS, Lucas Carvalho dos; MARIANO, André de Lima e Silva; FLORÊNCIO, Isadora; BÁO, Sônia Nair; SILVA, Sebastião William da; SOUZA, Paulo Eduardo N.; AZEVEDO, Ricardo Bentes; MUEHLMANN, Luís Alexandre. Chlorophyll-loaded castor oil nanoemulsions exhibit photodynamic therapy efficacy against b16-f10 melanoma with low cytotoxicity toward hacat keratinocytes. Pharmaceuticals, [S. l.], v. 19, n. 7, p. 974, 2026. DOI: https://doi.org/10.3390/ph19070974. Disponível em: https://www.mdpi.com/1424-8247/19/7/974. Acesso em: 12 ago. 2026.pt_BR
dc.identifier.urihttps://doi.org/10.3390/ph19070974pt_BR
dc.identifier.urihttp://repositorio.unb.br/handle/10482/55639-
dc.language.isoengpt_BR
dc.publisherMDPI AGpt_BR
dc.rightsAcesso Abertopt_BR
dc.titleChlorophyll-loaded castor oil nanoemulsions exhibit photodynamic therapy efficacy against b16-f10 melanoma with low cytotoxicity toward hacat keratinocytespt_BR
dc.typeArtigopt_BR
dc.subject.keywordTerapia fotodinâmicapt_BR
dc.subject.keywordClorofilapt_BR
dc.subject.keywordNanoemulsãopt_BR
dc.subject.keywordMelanomapt_BR
dc.subject.keywordPele - câncerpt_BR
dc.rights.licenseThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.pt_BR
dc.identifier.doihttps://doi.org/10.3390/ph19070974pt_BR
dc.description.abstract1Background: Photodynamic therapy (PDT) is a promising minimally invasive approach for melanoma; however, many photosensitizers lose activity in aqueous media due to aggregation-induced quenching effects. Objectives: The aim of this study was to develop and characterize castor oil–based nanoemulsions containing chlorophyll (NFs-Chl) and to evaluate their in vitro photodynamic potential against melanoma cells (B16-F10), as well as their selectivity compared with human keratinocytes (HaCaT). Methods: NFs-Chl were prepared by spontaneous emulsification. Physicochemical characterization was carried out using dynamic light scattering (DLS), UV–Vis spectroscopy, FTIR, and Raman spectroscopy. In vitro assays included MTT for cell viability (IC50 determination), real-time cell proliferation (RealTime-Glo™), and cell migration analysis (scratch assay). All photodynamic treatments were performed under irradiation at 660 nm. Results: NFs-Chl exhibited homogeneous nanometric sizes (≈24–31 nm) and a low polydispersity index (≈0.25–0.40), indicating a narrow size distribution. UV–Vis spectra confirmed the preservation of the characteristic absorption peaks of chlorophyll after encapsulation. In B16-F10 cells, NFs-Chl associated with PDT significantly reduced cell viability and metabolic activity over 48 h. Furthermore, NFs-Chl inhibited the migratory capacity of B16-F10 cancer cells. Cell migration assays revealed a clear inhibition of B16-F10 cell migration following treatment with NFs-Chl + PDT. Conclusions: Encapsulation of chlorophyll into castor oil nanoemulsions protected the photosensitizer, improved its cellular delivery, and enhanced its photodynamic cytotoxic effect against melanoma cells, while relatively preserving normal keratinocytes in vitro.pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4806-9192pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-9929-0201pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-8013-8250pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-5665-6349pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-9873-3098pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-4483-9687pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4289-8678pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-2137-9588pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-4711-7583pt_BR
dc.contributor.affiliationUniversity of Brasília, Institute of Biological Sciences, Department of Genetics and Morphologypt_BR
dc.contributor.affiliationUniversity of Brasília, Institute of Physics, Optical Spectroscopy Laboratorypt_BR
dc.contributor.affiliationUniversity of Brasília, Institute of Biological Sciences, Department of Genetics and Morphologypt_BR
dc.contributor.affiliationUniversity of Brasília, Institute of Chemistrypt_BR
dc.contributor.affiliationOptical Spectroscopy Laboratory, Institute of Physics, Darcy Ribeiro University Campus, University of Brasília, Brasília 70910-900, Brazilpt_BR
dc.contributor.affiliationUniversity of Brasília, Institute of Biological Sciences, Cellular Biology Departmentpt_BR
dc.contributor.affiliationUniversity of Brasília, Institute of Biological Sciences, Cellular Biology Departmentpt_BR
dc.contributor.affiliationUniversity of Brasília, Institute of Physics, Optical Spectroscopy Laboratorypt_BR
dc.contributor.affiliationUniversity of Brasília, Institute of Physics, Optical Spectroscopy Laboratorypt_BR
dc.contributor.affiliationUniversity of Brasília, Institute of Biological Sciences, Department of Genetics and Morphologypt_BR
dc.contributor.affiliationUniversity of Brasilia, Faculty of Health Sciences and Technologiespt_BR
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