Título :	Restoration of myogenesis in ALS-myocytes through miR-26a-5p-mediated Smad4 inhibition and its impact on motor neuron development
Autor :	Peggion, Caterina Bonadio, Raphael Severino Stella, Roberto Scalabrin, Silvia Pasetto, Laura Millino, Caterina Camporeale, Laura Pacchioni, Beniamina Bonetto, Valentina Bertoli, Alessandro Cagnin, Stefano Massimino, Maria Lina
metadata.dc.identifier.orcid:	https://orcid.org/0000-0002-9967-2629 https://orcid.org/0000-0002-4832-2776 https://orcid.org/0000-0003-1462-4423 https://orcid.org/0009-0000-3604-8792 https://orcid.org/0000-0003-0319-4448 https://orcid.org/0000-0003-0456-2054 https://orcid.org/0000-0003-1202-0191
metadata.dc.contributor.affiliation:	University of Padova, Department of Biology University of Padova, Department of Biology Istituto Zooprofilattico Sperimentale delle Venezie, Department of Chemistry University of Padova, Department of Biology Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Research Center for ALS University of Padova, Department of Biology Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Research Center for ALS University of Padova, Department of Biology Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Research Center for ALS University of Padova, Department of Biomedical Sciences University of Padova, Padova Neuroscience Center National Research Council (CNR), Section of Padova, Neuroscience Institute University of Padova, Department of Biology University of Padova, CIR-Myo Myology Center National Research Council (CNR), Section of Padova, Neuroscience Institute
Assunto::	RNAs não-codificadores Esclerose lateral amiotrófica Doenças neuromusculares Músculo esquelético
Fecha de publicación :	30-may-2025
Editorial :	Elsevier
Citación :	PEGGION, Caterina et al. Restoration of myogenesis in ALS-myocytes through miR-26a-5p-mediated Smad4 inhibition and its impact on motor neuron development. Molecular Therapy: Nucleic Acids, v. 36, n. 3, e102581, 2025. DOI: https://doi.org/10.1016/j.omtn.2025.102581. Disponível em: https://www.sciencedirect.com/science/article/pii/S2162253125001350?via%3Dihub. Acesso em: 27 jan. 2026.
Abstract:	Amyotrophic lateral sclerosis (ALS) is the most common adult-onset paralytic disorder, characterized primarily by a progressive loss of motor neurons (MNs) in which degeneration skeletal muscle involvement has been demonstrated. Skeletal muscle is a plastic tissue that responds to insults through proliferation and differentiation of satellite cells. Skeletal muscle degeneration and regeneration are finely regulated by signals that regulate satellite cell proliferation and differentiation. It is known that satellite cell differentiation is impaired in ALS, but little is known about the involvement of microRNAs (miRNAs) and their role in intercellular communication in ALS. Here we demonstrated impaired differentiation of satellite cells derived from ALS mice related to the impairment of myogenic p38MAPK and protein kinase A (PKA)/pCREB signaling pathways that can be regulated by miR-882 and -134-5p. These miRNAs participate in autocrine signaling in association with miR-26a-5p that, secreted from wild-type (WT) and captured by ALS myoblasts, enhances ALS-related myoblast differentiation by repressing Smad4-related signals. Moreover, miR-26a-5p and -431-5p work in a paracrine way ameliorating motoneuron differentiation. These findings emphasize the need to better understand intercellular communication and its role in ALS pathogenesis and progression. They also suggest that miRNAs could be targeted or used as therapeutic agents for myofiber and MN regeneration.
metadata.dc.description.unidade:	Instituto de Ciências Biológicas (IB) Departamento de Genética e Morfologia (IB GEM)
Licença::	This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
DOI:	https://doi.org/10.1016/j.omtn.2025.102581
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