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Title: Faster cryptococcus melanization increases virulence in experimental and human cryptococcosis
Authors: Sousa, Herdson Renney de
Oliveira Júnior, Getúlio Pereira de
Frazão, Stefânia de Oliveira
Gorgonha, Kaio César de Melo
Rosa, Camila Pereira
Melgaço Garcez, Emãnuella
Lucas Júnior, Joaquim
Correia, Amabel Fernandes
Freitas, Waleriano Ferreira de
Borges, Higor Matos
Alves, Lucas Gomes de Brito
Paes, Hugo Costa
Trilles, Luciana
Lazera, Márcia dos Santos
Teixeira, Marcus de Melo
Pinto Júnior, Vitor Laerte
Felipe, Maria Sueli Soares
Casadevall, Arturo
Pereira, Ildinete Silva
Albuquerque, Patrícia
Nicola, André Moraes
metadata.dc.identifier.orcid: https://orcid.org/ 0000-0001-6940-401X
https://orcid.org/ 0000-0002-1598-4191
https://orcid.org/ 0000-0002-8369-8680
https://orcid.org/ 0000-0002-8369-8680
https://orcid.org/ 0000-0001-8932-3954
https://orcid.org/ 0000-0003-1763-3464
https://orcid.org/ 0000-0003-0556-5310
https://orcid.org/ 0000-0002-9402-9167
https://orcid.org/ 0000-0001-8160-7784
Assunto:: Cryptococcus neoformans
Criptococose
Virulência (Microbiologia)
Melanina
Fagocitose
Issue Date: 12-Apr-2022
Publisher: MDPI
Citation: SOUSA, Herdson Renney de et al. Faster cryptococcus melanization increases virulence in experimental and human cryptococcosis. Journal of Fungi, v. 8, n. 4, 393, 2022. DOI: https://doi.org/10.3390/jof8040393. Disponível em: https://www.mdpi.com/2309-608X/8/4/393. Acesso em: 08 jun. 2022.
Abstract: Cryptococcus spp. are human pathogens that cause 181,000 deaths per year. In this work, we systematically investigated the virulence attributes of Cryptococcus spp. clinical isolates and correlated them with patient data to better understand cryptococcosis. We collected 66 C. neoformans and 19 C. gattii clinical isolates and analyzed multiple virulence phenotypes and host–pathogen interaction outcomes. C. neoformans isolates tended to melanize faster and more intensely and produce thinner capsules in comparison with C. gattii. We also observed correlations that match previous studies, such as that between secreted laccase and disease outcome in patients. We measured Cryptococcus colony melanization kinetics, which followed a sigmoidal curve for most isolates, and showed that faster melanization correlated positively with LC3-associated phagocytosis evasion, virulence in Galleria mellonella and worse prognosis in humans. These results suggest that the speed of melanization, more than the total amount of melanin Cryptococcus spp. produces, is crucial for virulence.
Licença:: Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/)
DOI: https://doi.org/10.3390/jof8040393
Appears in Collections:FMD - Artigos publicados em periódicos e preprints

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