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Title: Paracoccidioides HSP90 can be found in the cell surface and is a target for antibodies with therapeutic potential
Authors: Moura, Ágata Nogueira D’Áurea
Oliveira, Diane Sthefany Lima de
Paredes, Verenice
Rocha, Leticia Barboza
Oliveira, Fabiana Freire Mendes de
Lessa, Gustavo Meirelles
Riasco Palacios, Juan Fernando
Casadevall, Arturo
Albuquerque, Patrícia
Felipe, Maria Sueli Soares
Piazza, Roxane Maria Fontes
Nicola, André Moraes
Assunto:: Anticorpos monoclonais
Issue Date: 2020
Publisher: MDPI
Citation: MOURA, Ágata Nogueira D’Áurea. Paracoccidioides HSP90 can be found in the cell surface and is a target for antibodies with therapeutic potential. Journal of Fungi, v. 6, n. 4, 193, 2020. DOI: Disponível em: Acesso em: 19 maio 2021.
Abstract: Paracoccidioidomycosis (PCM) is one of the most frequent systemic mycoses in Latin America. It affects mainly male rural workers in impoverished regions, and the therapy can last up to two years or use drugs that are very toxic. Given the need for novel safe and effective approaches to treat PCM, we have been developing monoclonal antibodies (mAbs) that could be used not only to block specific fungal targets, but also modulate the host’s antifungal immunity. In this work we show the generation of and promising results with an mAb against Heat Shock Protein (HSP)90, a molecular chaperone that is an important virulence factor in fungi. Using recombinant Paracoccidioides lutzii (Pb01) and P. brasiliensis (Pb18) HSP90 proteins produced in E. coli, we immunized mice and generated polyclonal antibodies and an IgG1 hybridoma mAb. The proteins were very immunogenic and both the polyclonal serum and mAb were used in immunofluorescence experiments, which showed binding of antibodies to the yeast cell surface. The mAb successfully opsonized P. lutzii and P. brasiliensis cells in co-incubations with J774.16 macrophage-like cells. Our results suggest that this mAb could serve as the basis for new immunotherapy regimens for PCM.
Licença:: Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (
Appears in Collections:CEL - Artigos publicados em periódicos
FMD - Artigos publicados em periódicos e preprints
PGCTS - Artigos publicados em periódicos

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