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Title: A mouse variable gene fragment binds to DNA independently of the BCR context : a possible role for immature b-cell repertoire establishment
Authors: Maranhão, Andrea Queiroz
Costa, Maria Beatriz Walter
Guedes, Leonardo
Moraes-Vieira, Pedro Manoel
Raiol, Tainá
Brígido, Marcelo de Macedo
Assunto:: Reações antígeno-anticorpo
Maturação celular
Issue Date: 2013
Publisher: Plos One
Citation: MARANHÃO, Andrea Queiroz et al. A mouse variable gene fragment binds to DNA independently of the BCR context: a possible role for immature b-cell repertoire establishment. Plos One, v. 8, n. 9, p. e72625, 2013. Disponível em: < >. Acesso em: 14 dez. 2016.
Abstract: B-cell maturation occurs in several steps and requires constant stimulus for its continuing development. From the emergence of the pre-B-cell receptor, signal transduction stimulates and supports B-cell development. Current viewpoints indicate that both positive selection pressure for autoantigens and tonic signaling constitutively stimulate B-cell maturation. In this work, we tested for the presence of a putative DNA binding site in a variable gene segment in a germline configuration, independently of VDJ recombination. After a survey of the public antibody databases, we chose a single mouse heavy variable gene segment that is highly represented in anti-nucleic acid antibodies and tested it for ssDNA binding. A phage display approach was used to search for intrinsic binding to oligo deoxythymidine. The results revealed that binding to an antigen can be influenced by the use of a specific DNA binding V gene segment. Our data support the idea that some variable genes have intrinsic reactivity towards specific types of endogenous autoantigens, and this property may contribute to the establishment of the immature B-cell repertoire.
Licença:: Plos One - Copyright: 2013 Maranhão et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Fonte: Acesso em: 14 dez. 2016.
Appears in Collections:CEL - Artigos publicados em periódicos

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