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Título : Avaliação de protocolos de inseminação artificial em tempo fixo para bovinos Curraleiro Pé-Duro
Otros títulos : Insemination protocols of artificial evaluation in fixed time for cattle Curraleiro Pé-Duro
Autor : Santos, Renato dos
Orientador(es):: Ramos, Alexandre Floriani
Assunto:: Animais - melhoramento genético
Bovino - reprodução
Bovino - fisiologia veterinária - embriologia
Inseminação artificial - zootecnia
Fecha de publicación : 23-ago-2016
Citación : SANTOS, Renato dos. Avaliação de protocolos de inseminação artificial em tempo fixo para bovinos Curraleiro Pé-Duro. 2016. xvi, 52 f., il. Dissertação (Mestrado em Ciências Animais)—Universidade de Brasília, Brasília, 2016.
Abstract: This study aimed to determine the physiological response of locally adapted females Curraleiro Pé-Duro race under different synchronization protocols and ovulation induction, evaluating the efficiency of these, as well as the determination of ovulation time, ovulatory follicle size, emergency wave and finally the pregnancy rate of females submitted to TAI programs. In one experiment, all females (n=12) on day zero (D0) received an intravaginal device containing 1g of progesterone (P4) which remained for eight days and 2 mg of estradiol benzoate (EB) intramuscular (IM). After that the animals were randomly divided into two groups; 8D treatment (n=6) and 9D treatment (n=6). On removal day of the implant P4 (D8) cows in the 8D group received IM 150 μg of Sodium D-cloprostenol, 300 IU eCG and 1 mg of EB. In 9D treatment group the cows had the implant removed in D8 and received IM 150 μg of D-cloprostenol Sodic and 300 IU eCG, and in contrast to the control group, the animals received IM in D9 1mg EB. The treatments differed among themselves as to the BE application day. For the second experiment, all animals (n=11) in D0 received the same pharmacological treatment of the previous experiment and were randomly divided into two groups; 9D treatment (n=5) and P4D9 treatment (n=6). The animals 9D treatment group had P4 implant removed in D8 and received IM 150 μg of D-cloprostenol Sodic and 300 IU eCG, and D9 received IM, 1mg EB. In contrast the P4D9 treatment group the females received D8 IM 150 μg of D-cloprostenol Sodium and 300 IU eCG and D9 were removed P4 implant followed by IM administration of 1mg of EB. The treatments differed on the withdrawal time of P4 implants. In both experiments, on the P4 implant removal day was also initiated ovarian ultrasound assessment every 24h up to 24h after removal of the P4 implant. After this period, the evaluations were performed every 8 hours for up to 96 hours in the attempt to monitor from the FD to the ovulation. Experiments one and two occurred in crossover format. For the third experiment we used the protocols for the treatment 8D and P4D9 to TAI. Where, for the 8D treatment group (n=43) insemination occurred in the period of 40/42 hours and P4D9 for the treatment group (n=45) 24/26 hours after removing the P4 implant. For the experiment one, the 8D and 9D treatments led to a sync rate of the dominant follicle 92% (11/12) and 83% (10/12) and ovulation rate of 73% (8/11) and 100% (10/10), respectively. There was no effect of treatments on group and interaction day VS group (P> 0.05) in follicular development, only daily growth (P<0.05). The time of ovulation was early in the 8D treatment and variation of ovulation tended to differ (P=0,06). In the second experiment, the treatments 9D and P4D9 led to a sync rate of the dominant follicle 72% (8/11) and 100% (11/11) and ovulation rate of 87,5% (7/8) and 91% (10/11), respectively. For both experiment there was no difference in the luteal volume (P>0,05). As for the three experiments where animals were submitted to TAI, it is found to 8D treatment group and P4D9 treatment group pregnancy rates of 23% (10/43) and 20% (9/45) respectively (P>0,05), concluding that there is still need for more studies on the reproductive physiology of females Curraleiro Pé-Duro, is the hormonal and ultrasonographic control of follicular development, or the development of new methodologies in TAI specific protocols for animals of this breed.
metadata.dc.description.unidade: Faculdade de Agronomia e Medicina Veterinária (FAV)
Descripción : Dissertação (mestrado)—Universidade de Brasília, Faculdade de Agronomia e Medicina Veterinária, 2016.
metadata.dc.description.ppg: Programa de Pós-Graduação em Ciências Animais
Licença:: A concessão da licença deste item refere-se ao termo de autorização impresso assinado pelo autor com as seguintes condições: Na qualidade de titular dos direitos de autor da publicação, autorizo a Universidade de Brasília e o IBICT a disponibilizar por meio dos sites www.bce.unb.br, www.ibict.br, http://hercules.vtls.com/cgi-bin/ndltd/chameleon?lng=pt&skin=ndltd sem ressarcimento dos direitos autorais, de acordo com a Lei nº 9610/98, o texto integral da obra disponibilizada, conforme permissões assinaladas, para fins de leitura, impressão e/ou download, a título de divulgação da produção científica brasileira, a partir desta data.
DOI: http://dx.doi.org/10.26512/2016.02.D.21317
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