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Title: Methods of endotoxin removal from biological preparations : a review
Authors: Batista, Pérola de Oliveira Magalhães Dias
Lopes, André Moreni
Mazzola, Priscila Gava
Yagui, Carlota de Oliveira Rangel
Penna, Thereza Christina Vessoni
Pessoa Júnior, Adalberto
Assunto:: Endotoxina
Microbiologia farmacêutica
Contaminação - alimentos
Issue Date: 2007
Publisher: Faculty of Pharmacy & Pharmaceutical Sciences - Katz Centre for Pharmacy & Health Research - Canadá
Citation: MAGALHÃES, Pérola O. et al. Methods of endotoxin removal from biological preparations: a review. Journal of Pharmacy & Pharmaceutical Sciences, Edmonton, v.10, n.3, p.388-404, 2007. Disponível em: <>. Acesso em: 24 out. 2013.
Abstract: ABSTRACT
PURPOSE: Endotoxins, also called lipopolysaccharides (LPS), are major contaminants found in commercially available proteins or biologically active substances, which often complicate study of the biological effects of the main ingredient. The presence of small amounts of endotoxin in recombinant protein preparations can cause side effects in host organism such as endotoxin shock, tissue injury, and even death. Due to these reactions, it is essential to remove endotoxins from drugs, injectables, and other biological and pharmaceutical products. An overview of this subject is provided by this article. METHODS: An extensive review of literature with regard to methods for removal of endotoxin from biotechnological preparations was carried out. RESULTS: A short history of endotoxin is presented first. This is followed by a review of chemical and physical properties of endotoxin and its pathophysiological effects when the body is exposed to LPS excessively or systemically. The techniques of endotoxin determination and interaction of endotoxin with proteins is also presented, taking into consideration the established techniques as well as the state of the art technology in this field. A review of techniques of endotoxin mentioned with relatively high protein recoveries; however, special attention is given to two-phase aqueous micellar systems, which are valuable tools for endotoxin removal from pharmaceutical proteins on a small scale because they provide a mild environment for biological materials. CONCLUSIONS: Efficient and cost-effective removal of endotoxins from pharmaceutical and biotechnology preparations is challenging. Despite development of novel methods, such as the two- phase aqueous micellar systems, in recent years, more research is needed in this field.
Licença:: Copyright Notice: Submitted manuscripts must not be published previously or be currently under consideration for publication elsewhere. Acceptance of a manuscript for publication in the Journal is with the authors' approval of the terms and conditions of the Creative Commons copyright license Creative Common license (Attribution-ShareAlike) License. Direitos Autorais: Os manuscritos submetidos não deve ser publicado anteriormente ou estar atualmente sob consideração para publicação em outro lugar. Aceitação de um manuscrito para publicação no Jornal é com a aprovação dos termos e condições da licença Creative Commons copyright licença Creative Common dos autores (Atribuição-Compartilhamento pela mesma licença). Fonte: Acesso em: 24 out. 2013.
Appears in Collections:FAR - Artigos publicados em periódicos

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